Madonna seemingly came out in a TikTok video shared on Sunday (9 October).
The star took part in one of the social media platform’s latest trends, which saw her try to throw a pair of pink knickers into a trash can while the words: “If I miss, I’m gay,” flashed up on screen.
Madonna, who was rocking bright pink hair and standing in a luxury bathroom, half-heartedly threw her underwear towards the bin, but missed.
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Her social media followers have interpreted Madonna’s clip as her coming out, with one person writing beneath the footage: “Did I just witness Madonna coming out? Good for her.”
Another added: “Welcome to the better side, queen,” while a third wrote: “I’m so happy for you!”
However, others were quick to point out that Madonna, who has spoken openly about sexual fluidity in the past, has already come out as bisexual.
One person commented: “Y’all late she been on our team,” while a second echoed: “Madonna has been an out bisexual for literally decades my dudes.”
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“Y'all Madonna has been out as bi longer than some of us have been alive,” added another TikToker.
The 'Vogue' hit-maker famously snogged Britney Spears and Christina Aguilera at the 2003 MTV Video Music Awards, but has never publicly dated a woman.
Back in 1990, MailOnline reports that Madonna said: “I think everybody has a bisexual nature. That’s my theory. I could be wrong,” when promoting her 'Justify My Love' video.
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Also, in a 1991 letter to supermodel Amanda Cazalet, which was made public by The Sun, Madonna said: "I think you are the most beautiful woman in the world. I’m dying to kiss you again. I fantasise about you all the time."
She continued: "I cannot explain my attraction — nevertheless it’s there. I’m attracted to very few women in this way."
However, the 'Like a Virgin' singer has never publicly claimed to be bisexual.
Tyla has approached Madonna’s representatives for comment.
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Back in August, Madonna spilled the tea on her sex life in a Q&A video, saying her favourite snack is 'big d**k'.
Asked what her favourite snack was, she quipped: "I can't remember his name... oh, no! Big d**k."
For a lot of the questions, she simply gave the one-worded answer: "Sex".
That answer applied to her greatest guilty pleasure, her zodiac sign, her current favourite obsession, what keeps her going, the secret to her success, and her life mantra. And we’re living for her honesty.